Reporter
GFP2

Part:BBa_I715020:Design

Designed by: Will DeLoache   Group: iGEM07_Davidson_Missouri_W   (2007-06-22)

Carboxyl Half of GFP (aka GFP2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 174


Design Notes

Half-GFP program in the PTC-100™ Programmable Thermal Controller:

  1. Step 1: 95° C 5 minutes
  2. Step 2: 95° C 1 minute
  3. Step 3: 47.24° C 1 minute
  4. Step 4: 72° C 30 seconds
  5. Repeat steps 2-4 29X

Primers:

  • GFP2 Forward 5’GCATGAATTCGCGGCCGCTTCTAGATAAGAATGGAATCAAAGTTAA 3’
  • GFP2 Reverse 5’GCATCTGCAGCGGCCGCTACTAGTTTATTATTTGTATAGTTCATCCATG 3’


Source

[http://www.bio.davidson.edu/Courses/Immunology/Students/spring2006/Acker/karen_project.html summary web page]


References